![]() ![]() Have you already mastered western blot analysis? Check out our training programs on antibody basics, fluorescent imaging, IHC, flow cytometry, and ChIP. Troubleshooting the most common problems in western blotīoost your essential skills with our western blot training.Western blotting with phosphorylated proteins and histones.Implementing fluorescent western blot protocol Dot blot is a technique for detecting, analyzing, and identifying proteins, similar to the western blot technique, but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate.Including the appropriate controls for western blot analysis.Selecting the right antibodies for your western blot experiment.Basic principles of western blot analysis.You’ll also have a good understanding of: Training valueĪt the end of this online training, you’ll be fully equipped to run the entire western blot experiment. No band was observed when PKC alpha knockout samples were examined. ab32376 was shown to specifically react with PKC alpha in wild-type HAP1 cells. Red - loading control, ab8245, observed at 37 kDa. Western blot with Anti-PKC alpha antibody ( ab32376 ). Upon training completion, you can test your knowledge with our final quiz and get a certificate.Įnroll now and instantly access all training materials.ĭiscover your best work with straightforward blotting. We’ll also guide you through a detailed fluorescent western blot protocol and share some troubleshooting tips. Part 4 explains the advantages of fluorescent western blotting and how to make the switch from chemiluminescence to fluorescence.You’ll also gain access to step-by-step western blot protocols for phosphorylated proteins and histones. Part 3 helps you troubleshoot the most common problems you might encounter in western blot.Part 2 takes you through all steps of western blot protocol, including gel electrophoresis, protein transfer and visualization, and membrane stripping for restaining.You’ll also get recipes for the essential western blot buffers and solutions. ![]() ![]() Part 1 guides you through western blot basics, from choosing the right antibody and the appropriate controls to sample preparation.Our western blot training is divided into 4 parts: From basic principles and essential steps of the western blot protocol to optimization and more advanced techniques, our online training will teach you how to run your western blot experiments and help troubleshoot them. Whether you are questioning what western blotting is or trying to optimize your next experiment, our training has got you covered. ![]()
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